The sperm freeze procedure freezes sperm cells to preserve them for future use. To begin, the embryologist verifies that the sample belongs to the appropriate patient and evaluates the volume of the sample, along with the sperm concentration, motility and progression. The sample is then placed into a tube and combined with a sperm cryopreservation media.
Once the sample is equilibrated, it is evenly distributed among pre-labeled cryopreservation vials, including a test thaw vial, and placed into a liquid nitrogen vapor, and later, into liquid nitrogen storage. Once the samples have been frozen, the test vial is thawed to ensure the survivability of the sperm. Using this sperm freezing process, the sperm can be safely stored in the liquid nitrogen substance for many years.